A study on Genetic Polymorphism of GPT loci by FLDAS-PCR and PCR-RFLP
- VernacularTitle:用FLDAS-PCR和PCR-RFLP检测汉族人群GPT的多态性
- Author:
Caixia ZHAO
;
Hui CHEN
;
Haiyan SONG
- Publication Type:Journal Article
- Keywords:
Forensic science;
Glutamate pyruvate transaminase (GPT);
Fragment length discrepant allele specific PCR (FLDAS-PCR);
PCR restriction fragment length polymorphism (PCR-RFLP)
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new allele specific PCR method for GPT typing: and study GPT polymorphism by FLDAS-PCR and PCR-RFLP. Method 248 unrelated individuals of Han were genotyped using FLDAS-PCR and PCR-RFLP. Results Three genotypes were identified with DP of 0.631 in Han population. Typing results by FLDAS-PCR were completely consistent with those by PCR-RFLP. The allele frequencies were GPT * 1 = 0.5423 and GPT * 2 = 0.4577. The population data fitted the Hardy-Weinberg law. Conclusion GPT-FLDAS-PCR and GPT-RFLP method is useful for forensic identification.
