A sensitive radioimmunoassav for morphine in a single hair
- VernacularTitle:测定单根毛发中吗啡含量的放射免疫方法
- Author:
Yang RONG
;
Licheng SHAO
;
Zhijie SHI
- Publication Type:Journal Article
- Keywords:
Forensic toxicological analysis;
Morphine;
Hair;
Radioimmunoassay
- From:
Chinese Journal of Forensic Medicine
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The paper described a convenient and sensitive radioirnmunoassav method which can be used to measure the amount of morphine in a single hair. Method Succinvl-eggalbumin-morphine was used as immunogen to immunize New Zealand rabbits for obtaining high quality antiserum. The morphine- labeled with Iodine-125 was purified by HPLC. The radioimmunoassay method was established and the amount of morphine in a single hair was detected. Results The affinity constant of antibody is 3.25 ? 10~11L/M. The radiochemical purity of I-mophine is 95% and the specific radioactivity is 112?Ci/?g. The detection limit for morphine (3SD from zero-dose binding) is 0.01ng/ml. Five normai person's single hair and five addict's were detected . The length of these specimens is 9-24cm and the weight of them 0.7- 2. 1mg. The amount of morphine in the hair of normai person is 1. 75 ? 0.37ng/mg (x ? 5) and that of the addictos'is 471 ?204ng/mg (x ? s). Conclusion This method can be employed for accurate mea-surement of the amount of morphine in a single hair and the result can be adopted to indicate the probability of illicit-drug use.
