Determination of the Contents of Rhein and Emodin in Jianfei Jiangzhi Tablets by HPLC
- VernacularTitle:高效液相色谱法测定减肥降脂片中大黄酸、大黄素的含量
- Author:
Cheng GUO
;
Wenji WANG
;
Chunxia JIANG
;
Gaolin LIU
;
Chun ZHANG
- Publication Type:Journal Article
- Keywords:
Jianfei Jiangzhi tablet;
rhein;
emodin;
HPLC;
content
- From:
China Pharmacy
1991;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for determining the contents of rhein and emodin in Jianfei Jiangzhi tablets.METHODS:Stationary phase was Waters Symmetry C 18 (250mm?4.6mm;5?m),mobile phase was menthol-0.01%H 3 PO 4 (90∶10),detection wavelength440nm,flow rate1.0ml/min and column temperature30℃.RESULTS:There was good linearity in range of0.108?g～0.468?g for rhein and in range of0.180?g～0.480?g for emodin.The average recoveries of rhein and emodin were99.7%and98.6%respectively;RSD were1.93%and2.19%respectively.CONCLUSION:This method is rapid and accurate.It can be applied to effective control of the quality of Jianfei Jiangzhi tablet.
