The expression analysis of EGF and EGFR in esophageal restenosis tissue after stenting
- VernacularTitle:食管支架术后再狭窄组织中表皮生长因子及其受体的表达分析
- Author:
Xinjian WAN
;
Zhaoshen LI
;
Guoming XU
- Publication Type:Journal Article
- Keywords:
Esophageal stent;
Restenosis;
Epidermal growth factor;
Receptors, epidermal growth factor;
Gene expression
- From:
Chinese Journal of Digestive Endoscopy
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of EGF and EGFR in restenosis tissue of animal e-sophagus after several times of stenting in esophagus, and their relation with esophageal restenosis . Methods Animal models of esophageal stenosis after stenting were made from 16 adult healthy dogs, and allocated into 4 groups to take restenosis tissue as experimental samples at week 1,2,4 and 8. In addition, other 6 normal dogs served as control. To study the expression of EGF and EGFR at protein and mRNA level in these samples by immunohistochemistry ( SABC) and RT-PCR. Results At week 1 and 2, EGF and EGFR clearly expressed, the expressing level increased greatly as compared with the normal controls, the expression of EGF and EGFR existed almost in all the tissues. At week 4 the expression lessened greatly, and finally at week 8 disappeared. The positive cells for EGF were mainly macrophages, some vascular endothelial cells and smooth muscle cells, the positive cells for EGFR were mainly macrophages, lymphocyte and Fb. Conclusion After esophageal stenting, lymphocyte, macrophage and Fb in the local tissues were closely related to restenosis, and served as the important regulatory cells in the pathogenesis of restenosis.
