Measurement of the Rectum Carcinoma's Sensitivity to the Anticancer Medicine by Nude-mouse Subrenal Capsule Assay and MTT Assay
- VernacularTitle:裸鼠肾包膜下移植法与四唑盐比色法检测直肠癌对抗癌药物的敏感性
- Author:
Chunhong QIN
;
Yongguo LI
;
Hanxian LI
- Publication Type:Journal Article
- Keywords:
SRCA;
MTT assay;
Nude mouse;
Rrectum carcinoma;
Chemosensitivity
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the difference between subrenal capsule assay (SRCA) and monotetrazolium (MTT) assay in anticancer chemosensitivity test and explore the possible cause of bringing the difference,in order to provide theoretical basis for clinical practice.Methods SRCA and MTT assay were carried out with fresh rectum carcinoma in three groups:5-FU,amethopterin(MTX) and mitomycin (MMC).The day 1,3,5 after SRCA,every subgroups was injected with 5-FU ,MMC and MTX respectively and the RR was observed at the sixth day;In MTT assay ,the drug,s concentration was 1/10 PPC of each group's drug and the IR was worked out at the fourth day after MMT assay.Results According to the result of chemosensitivity in the 60 groups,20 groups of them were accorded with,other 40 groups were not.The positive and negative according rate of tumor's sensitivity to drug was 33 3% and there was statistical difference between the two methods.Conclusions There were considerable difference between SRCA and MTT assay in anticanaer chemosensitivity test.
