Observation on Biological Character of Cultivated Cornea Limbal Epithelial Cells
- VernacularTitle:体外培养的角膜缘上皮细胞生物学特性
- Author:
Xinghua LIU
;
Meiling ZHU
;
Ti CHEN
- Publication Type:Journal Article
- Keywords:
Cornea limbal epithelial cells;
Culture;
Biological character
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe biological character iistcs of cultivated cornea limbal epithelial cells of rabbit.Methods Cultivated cornea limbal epithelial cells with tissue inoculation were observed by inverted microscope every two days; ultrastructure of cell was observated by electron microscope; Proliferative ability of cell was examined by flow cytometry; the cell growth curve was examined by monotetrazolium(MTT) colorimetry.Results Cultivated limbal cornea epithelial cells formed monolayer at six day,the cultivated conea limbal epithelial cells had high metabolism and proliferative ability, growth of cornea limbal epithelial cells reached climax after 4 days by passage culture. Conclusion Cultivated limbal epithelial cells during the first and second growth generation can keep cornea epitheliam property and high proliferative ability.
