The Study on the Levels of HBV-DNA in Culture Supernatants of Peripheral Blood Mononuclear Cells From HBeAg Positive Carriers
- VernacularTitle:乙肝病毒携带者外周血单个核细胞培养上清液HBV-DNA定量检测意义
- Author:
Minning SONG
;
Meilan LUO
;
Wenqi HUANG
- Publication Type:Journal Article
- Keywords:
PBMC;
Carriers of HBeAg positive;
HBV-DNA;
FQ-PCR;
Fluorescence quantitative PCR
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
5000 copy/ml were both significantly higher than those in the controls.The levels of HBV-DNA positive in cultured supernatants of PBMC from suffering grave hepatitis B were higher than those in the sera by qualitative PCR of the same period.They were similar between the positive rate of quantitative PCR of HBV-DNA in sera and FQ-PCR in cultured supernatants of PBMC from hepatitis B types.They were not obviously related with the levels of ALT and TBiL.Conclusions The IL-12 and ? IFN secretion increased when the HBV-DNA of PBMC from carriers of HBeAg were at high-reproducing state.The HBV-DNA positive rate in cultured supernatants of PBMC of grave hepatitis detected by FQ-PCR was higher than that in sera by qualitative PCR.
