The Study of DNA in Middle Ear Effusions
- VernacularTitle:中耳分泌液中DNA分析
- Author:
Peizhong LI
;
Mingling QIU
;
Hua ZHAO
- Publication Type:Journal Article
- Keywords:
Middle ear effusion;
Otitis media with effusion in children;
Specific viscosity;
DNA
- From:
Journal of Chinese Physician
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether or not the bacterial DNA which was detected by PCR comes from viable bacteria.Methods Observe the effection of middle ear effusion(MEE) on DNA viscosity and enzymatic digestion of DNA.Results The middle ear effusion and DNA are stable and DNase 1 rapidly digests DNA,The effusion does not seem to degrade DNA.The middle ear effusion signifcantly inhibits DNase 1.Conclusions Middle ear effusion provides an inhibition of the enzymatic digestion of purified DNA.Thus any DNA found in effusion by PCR techniques could well be fossilized remains and chronic otitis media with effusion may not be the bacterial infection that recent studies have suggested.
