IL-1?mRNA Expression and Protein Secretion After Ultraviolet Irradiation in Human Keratinocyte Originated SCC12F Cells
- VernacularTitle:鳞状细胞癌细胞系12F细胞中白介素1?mRNA的表达及对紫外线照射的反应
- Author:
Dan LUO
;
Wei MIN
- Publication Type:Journal Article
- Keywords:
Keratinocytes;
Tumor cells,cultured;
Interleukin-1;
Ultraviolet rays
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate IL-1?mRNA expression and protein secretion after ultraviolet irradiation in human keratinocyte(KC)originated SCC12F cells.Methods Expression of IL-1?mRNA was detected by Northern blot and the secretion level of IL-1?protein was analyzed by ELISA.Results There was a spontaneous expression of IL-1?mRNA in SCC12F cells in culture system of normal KC,which was increased along with the culture time,and reached the highest expression level in120hours.The secretion level of IL-1?protein was increased in a time-and dose-dependent manner in SCC12F cells after ultraviolet B(UVB)irradiation.Conclusions SCC12F cells may express IL-1?mRNA spontaneously under regular KC media.The secretion level of IL-1?protein shows both time-and dose-effect after UVB irradiation.
