Detection of Mycobacterial16S rRNA Gene and M.tuberculosis DNA in Skin Lesions of Sarcoidosis with Polymerase Chain Reaction
- VernacularTitle:用聚合酶链反应检测结节病皮损中分支杆菌16SrRNA基因和结核杆菌DNA
- Author:
Lin CAI
;
Jianzhong ZHANG
- Publication Type:Journal Article
- Keywords:
Sarcoidosis;
Mycobacterium;
Polymerase chain reaction
- From:
Chinese Journal of Dermatology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible role of mycobacterial infection in the pathogenesis of sarcoidosis.Methods DNA fragments of mycobacterial16S rRNA gene and the complex-specific insertion sequence IS6110of M.tuberculosis were detected by polymerase chain reaction(PCR)in formalin-fixed,paraffin-embeded skin biopsy specimens from12patients with sarcoidosis.Results Mycobacterial common sequence16S rRNA gene was found in6patients,among which5were further confirmed as M.tuberculosis,the other one was atypical mycobacteria.The IS6110DNA fragment amplified by PCR was confirmed by DNA sequencing.Conclusion The above findings suggest an aetiological role of mycobacterial infection,especially M.tuberculosis,in some patients with sarcoidosis.
