Method of Isolating Deiters Cell from Guinea Pig Cochlea and Criterion of Cell Judgement
- VernacularTitle:豚鼠耳蜗单离Deiters细胞分离方法及活性判定标准
- Author:
Chenkai DAI
;
Weijia KONG
- Publication Type:Journal Article
- Keywords:
Cochlea Deiters cell
- From:
Journal of Audiology and Speech Pathology
1998;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe the method of dissociating isolated Deiters cells of cochlear and its judging criterion for living Deiters cells.Methods Deiters cells were dissociated from guinea pig cochlea separately by collagenase,papain,trysin digestion followed by trituation.After that ,the Deiters cells were observed and counted.Results Isolated Deiters cell looked like a comma, which was divided into cell body, stem and Deiters phalanx. About 20 to 32 isolated Deiters cells were obtained from every two cochleae.The membrane was smooth and the birefringence existed; the position of the nucleus was normal; full phalanx and no particles with Brownian movement.Conclusion Substantial sum of Deiters cell with sound vitality can be obtained by all three enzymes digestion followed trituation;The criterion used is reliable.
