Preliminary study on induction of mouse embryonic stem cells into neural stem cells in vitro
- VernacularTitle:体外诱导小鼠胚胎干细胞分化为神经干细胞的实验研究
- Author:
Jing TAO
;
Jian GE
;
Bing HUANG
;
Zhichong WANG
;
Yan GUO
;
Keming YU
;
Huiyi CHEN
;
Xig CHEN
- Publication Type:Journal Article
- Keywords:
Embryonic stem cells;
Neural regeneration;
Mice
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the feasibility of inducing mouse embryonic stem cells into neural stem cells in vitro. METHODS: Embryonic body induced by retinoic acid and retinal m?ller cells were selected in neural stem cell-defined medium for 7 days, and the morphological changes were observed. The selected cells were stained immunocytochemically with anti-nestin, anti-BrdU antibodies, and their ability of expansion and differentiation were analyzed. RESULTS:Large amounts of neurospheres were derived from embryonic body in the selected medium on the 7th day, which could be passaged and differentiated, stained positive with nestin and BrdU, and expressed nestin, glutaminase and Brn-3 genes. CONCLUSION:Neural stem cells could be derived from embryonic body induced by RA and m?ller cells in the selected medium, which would offer an alternative to treat neuropathy such as glaucoma and retinal degeneration in the future.
