Evidence for high-frequent deletion of p53 gene in primary hepatocellular carcinoma by interphase dual fluorescence in situ hybridization
- VernacularTitle:原发性肝癌p53基因高频缺失的双色荧光原位杂交证据
- Author:
Yan FANG
;
Bijun HUANG
;
Qiwan LIANG
;
Huimei LI
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Genes, p53;
In situ hybridization;
Chromosomes, human, pair 17
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the frequency and pattern of deletion of p53 gene in primary hepatocellular carcinoma (HCC) and its clinical significance. METHODS: The interphase dual fluorescence in situ hybridization(FISH) technique was applied to detect loss of p53 gene in HCCs. RESULTS: The deletion of p53 gene was found in 68.0% of HCCs whereas no loss of p53 gene was detected in 40 mated normal liver specimens. Loss of p53 gene was closely related to tumor size and serum ?-fetoprotein(AFP) level in HCC patients ( P 0.05). The 2-year survival rate of postoperative HCC patients was significantly lower in the HCC cases with p53 gene deletion (25.6%) than those without p53 gene loss (69.6%) ( ? 2=11.463, P
