Role of PARP and caspase-3 in the airway epithelial injury induced by peroxynitrite
- VernacularTitle:多聚(ADP-核糖)聚合酶和半胱天冬蛋白酶-3在过氧亚硝基阴离子致气道上皮细胞损伤中的作用
- Author:
Ruijing ZHAO
;
Tienian ZHU
;
Yiling LING
;
Yanqun LI
;
Baoe SHAN
- Publication Type:Journal Article
- Keywords:
Airway;
Epithelium;
Apoptosis;
Caspases;
Asthma;
Poly-(ADP-ribose) polymerase
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the mechanism responsible for ONOO --induced the airway epithelial injury. METHODS: Effects of 3-aminobenzamide(3-AB), a poly-(ADP-ribose) polymerase(PARP) inhibitor, and Ac-DEVD-CHO, a caspase-3 inhibitor, on LDH release and apoptosis of cultured rat tracheal epithelial (RTE) cells induced by ONOO - were examined. The cleavage of PARP was analysed by Western blot. RESULTS: 3-AB inhibited the release of LDH induced by ONOO - partially, and had no effect on the apoptosis of RTE cells. Caspase-3 inhibitor Ac-DEVD-CHO obviously prevented the apoptosis of RTE cells induced by ONOO - in a dose-dependent manner. The cleavage of PARP was observed in the process of apoptosis of RTE cells induced by ONOO -. CONCLUSIONS: PARP activation represents one of the pathways of ONOO --mediated epithelial injury, and the excessive activation of PARP contributes to the necrosis in RTE cells induced by ONOO -. Cleavage of PARP by activated caspase-3 plays a crucial role in the apoptosis of RTE cells induced by ONOO -.
