Effect of cyclophosphamide on proliferation and apoptosis of rat mesangial cells in vitro
- VernacularTitle:环磷酰胺在体外对大鼠肾小球系膜细胞增殖及凋亡的影响
- Author:
Tong XIE
;
Ping WU
;
Deshen TANG
;
Limin JIANG
- Publication Type:Journal Article
- Keywords:
Cyclophosphamide;
Apoptosis;
Glomerular mesangial cells;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro. METHODS: GMC proliferation was detected by MTT method, GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis. The levels of Fas and Bcl-2 were also detected by immunohistology. RESULTS: The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased. CONCLUSION: CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level.
