Propofol and ketamine attenuate antigen-induced bronchoconstriction and microvascular leakage in rats
- VernacularTitle:异丙酚、氯胺酮对哮喘大鼠气道痉挛及气道渗出的作用
- Author:
Yong GUI
;
Junke WANG
;
Yanhong SUN
- Publication Type:Journal Article
- Keywords:
Propofol;
Ketamine;
Asthma;
Bronchoconstriction;
Airway microvascular leakage
- From:
Chinese Journal of Anesthesiology
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of propofol and ketamine on antigen-induced bronehoconstriction and microvascular leakage in rats.Methods Thirty SD rats of both sees weighing 150-200 g were sensitized with ovalbumin 1 mg, aluminium hydroxide 200 mg and devitalized Bordetella pertusis (6 ? 109 ) adminisered intraperitoneally 2 weeks before experiment. The animals were anesthetized with pentobarbital 30 mg ? kg-1 ip, tracheotomized and mechanically ventilated (VT = 10 ml? kg-1 , RR = 70 bpm) . The animals were randomly divided into 5 groups with 6 animals in each group: group I received normal saline iv group II propofol 50 mg?kg-1?h-1 iv group III propofol 100 mg?kg-1?h-1 iv group IV ketamine 50 mg?kg-1?h-1 iv; and group V ketamine 100 mg? kg-1? h-1 iv. Two minutes after propofol or ketamine administration Evan's blue 30 mg?kg-1 was given iv. Five minutes after dye injection ovalbumin 15 mg?kg-1 was injected iv to trigger asthmatic attack which was maintained for 30 min when airway pressure was measured. Then the animals were sacrificed by bleeding. Heart and lungs were removed for determination of lung coefficient( wet lung weight/body weight) , wet lung/dry lung ratio, lung extravascular water content [ (wet lung weight - dry lung weight/wet lung weight ? 100% ] and lung Evans blue content (formamide extraction method) . Results Propofol and ketamine significantly attenuated ovalbumin-induced bronchoconstiction( P
