Co-culture of lymphocytes and hepatocellular carcinoma cells induces specific cytotoxic T-lymphocyte lines
- VernacularTitle:淋巴细胞与肝癌细胞混合培养诱导特异性细胞毒T淋巴细胞的形成
- Author:
Junle LI
;
Xiaoming YE
;
Quanyong CHENG
- Publication Type:Journal Article
- Keywords:
Tumor-specific T cell;
Tumor adoptive immunotherapy;
Hepatocellular carcinoma;
Culture
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce highly active hepatocellular carcinoma (HCC) specific cytotoxic T-lymphocyte (H-S-CTL) lines by co-culture of lymphocytes and hepatocellular carcinoma cells. Methods H-S-CTL were established by co-culture of autologous HCC cells and lymphocytes, under stimulation of IL-1,IL-2,IL-4 and IL-6.The cytotoxic activity of H-S-CTL for target cells were detected by 51Cr release assay and indirect immunofluorescence assay. Results Compared with auto-LAK, H-S-CTL showed much higher antitumor activity of significantly increased CD3+, CD4+ and CD8+ cells. Conclusions H-S-CTL lines with massive proliferations and high antitumor activity can be successfully obtained by ex vivo co-culture under the stimulation of interlukins.
