In vitro killing of hepatocellular carcinoma by radio-sensitized suicide gene
- VernacularTitle:放射线敏感性自杀基因体外杀伤肝癌细胞的实验研究
- Author:
Yonggang FAN
;
Xuehao WANG
;
Xing L
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Gene therapy;
Gamma rays;
rRNA promoter
- From:
Chinese Journal of General Surgery
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the killing effect on hepatocellular carcinoma(HCC) cell lines by growth response-1(Egr-1) promoter activated herps simplex virus thymidine kinase (tk). MethodsPlasmid pET was constructed by fusing of Egr-1 promoter to the upstream of tk gene and transfect human hepatocellular carcinoma cell lines SMMC-7721(SMMC/ET) with lipofectamine as a delivery system. The cloned cells, after selected with G418, and exposure to ?-radiation by a 60Co source, were added with prodrug GCV. The viability of cell lines was observed. ResultsAfter irradiation, transfected cell lines (0.07?0.03) was killed by prodrug GCV at higher percentage compared with control group(0.88?0.12)(P
