Determination of the Content of Hydroxyproline in Liver Tissue by RP-HPLC in Rats with Hepatic Fi?brosis
- VernacularTitle:高效液相色谱法测定大鼠纤维化肝组织中羟脯氨酸含量
- Author:
Jing TIAN
;
Jian LU
- Publication Type:Journal Article
- Keywords:
hydroxyproline;
2,4-dinitrochlorobenzene;
hepatic fibrosis;
HPLC
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of hydroxyproline in liver tissue in rats with hepatic fibrosis.METHO_ DS:RP-HPLC was used with precolumn derivation by2,4-dinitrochlorobenzene(DNCB)in the Borax buffer solution for lh in boiling water.Acetonitrile-acetate buffer linear gradient elution was used.Detecting wavelength was360nm.RESULTS:Derivation of hydroxyproline could be separated from other amino acids and accurately determined.CONCLUSION:The method is rapid,accurate,and suitable for detection of biopsy samples obtained by liver puncture.
