Determination of the Concentration of Zaleplon in Human Plasma by HPLC
- VernacularTitle:高效液相色谱法测定人血浆中扎莱普隆的浓度
- Author:
Xiaobing DENG
;
Zhiling YANG
- Publication Type:Journal Article
- Keywords:
zaleplon;
HPLC;
fluorescence;
plasma;
concentration
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To build up a method for determining the concentration of zaleplon in human plasma by HPLC. METHODS:The Kromasil-C 8 column was used and mobile phase consisted of methanol-water(65∶35);the flow rate was1.0ml/min,fluorescence excitation wave-length was233nm;fluorescence emission wave-length was460nm.RESULTS:The calibration curve revealed linearity in the concentration range of0.42～73.92ng/ml(r=0.9999)with a regressive equation of Y=1.870?147.The average recovery was99.47%.The with-day and between-day RSDs were4.9%and4.73%respec?tively.CONCLUSION:The method is accurate,reliable and highly sensitive so it can be used in study of pharmacokinetics of zaleplon.
