Role of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Extracellular Matrix Remodeling in Rat Model of Chronic Obstructive Pulmonary Emphysema
- VernacularTitle:MMP-9和TIMP-1在大鼠肺气肿模型细胞外基质重塑中的作用
- Author:
Peifang ZHANG
;
Jianbao XIN
;
Lei PAN
- Publication Type:Journal Article
- Keywords:
Smoking;
Emphysema;
Metalloproteinase
- From:
Journal of Chinese Physician
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in extracellular matrix remodeling in rat model of chronic obstructive pulmonary emphysema .Methods Seventy Wistar rats were randomly subdivided into the control group and the smoking groups which were randomly subdivided into one month to six month smoking group again.The smoking groups were exposed to cigarette smoke for one to six months, The expressions of MMP-9 and TIMP-1 mRNA and proteins in lung tissue were detected by in situ hybridization and immunohistochemistry respectively.In lung tissue the expression of collagen Ⅳ was assessed by method of immunohistochemistry.Results Compared with the control group,the expressions of MMP-9 and TIMP-1 mRNA and proteins in lung tissue from the smoking groups were remarkable elevated(P
