The Antagonistic Effect of PGE_1 on TGF?_1 mRNA Expression and Rat Renal Interstitial Fibrosis Induced by Unilateral Ureleral Obstruction
- VernacularTitle:前列腺素E_1对输尿管梗阻大鼠肾间质纤维化及转化生长因子?_1 mRNA表达的影响
- Author:
Hong GUO
;
Miaozheng ZHU
;
Jian DUAN
- Publication Type:Journal Article
- Keywords:
Prostaglandin E 1;
Transforming growth factor? 1;
Tubulointerstitial fibrosis
- From:
Journal of Chinese Physician
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the possible role of PGE 1 in tubulointerstitial fibrosis formation. Methods Rat model of unilateral ureteral obstruction(UUO) was established.In situ hybridation(ISH) technique, morphological observation and biochemical analysis were applied to determine TGF? 1 mRNA expression, changes of tubulointerstitium and rencortical hydroxyproline content after used PGE 1. Results 3 days after UUO,the rats renal interstitial edema, a number of mononuclear cells recruitment. On day 14,tubule atrophy, interstitial proliferative fibrosis and area enlarged,hydroxyproline content,TGF? 1 mRNA expression increased, the peak value of the latter occurred early than that of hydroxyproline peak value and interstitial area enlargement.PGE 1 could down -regulate these changes.Conclusions The antagenistic effect of PGE 1 on renal interstitial fibrosis may be partly associated with down-regulating overexpression of TGF? 1 mRNA and inhibiting mononuclear cells recruitment.
