The protective effects of heat-shock response on the retinae of rats after retinal ischemic reperfusion injury
- VernacularTitle:热休克反应对大鼠视网膜缺血再灌注损伤的防御作用
- Author:
Bin GUO
;
Xinguang YANG
;
Li WANG
- Publication Type:Journal Article
- Keywords:
Ischemia;
Ocular hypertension;
Electroretinography;
Heat-shock response;
Heat-shock protein 70
- From:
Chinese Journal of Ocular Fundus Diseases
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo analyze the protective effects of heat-shock response on the retinae of the rats after retinal ischemic reperfusion injury. MethodTwenty Wistar rats (20 eyes) were divided into 4 groups: intracameral perfusion group (group P), intracameral perfusion after quercetin injection group (group P+Q), intracameral perfusion after heat shock group (group P+H), and intracameral perfusion after quercetin injection and heat shock group (group P+Q+H). According to the standard program established by International Society for Clinical Visual Electrophysiology, we recorded the results of the dark-adapted electroretinogram (D-ERG),oscillatory potentials (OPs),and light-adapted ERG (L-ERG) of the rats with intraocular hypertension after induced by heat shock response. The expressions of HSP 70 of the rats in all groups were observed by Western blotting.ResultsThe expression of HSP 70 of the rats in group P+H was the highest in all groups, but the expressions of HSP70 in group P+Q and P+Q+H were inhibited significantly. The amplitudes of a and b wave of ERG and O 2 wave of OPs decreased, and the delitescence of them were delayed significantly in rats after intracameral perfusion. The amplitude of b wave of D-ERG and O 2 wave of OPs in group P+H were higher than which in group P. Zero hour after perfusion, the amplitudes of all waves in group P+H increased significantly (P
