NF-?B site of promotor mediates nicotine-induced ICAM-1 expression in human umbilical vein endothelial cells
- VernacularTitle:顺式调控元件NF-?B在尼古丁诱导ICAM-1表达中的作用
- Author:
Weihui ZHOU
;
Xiangyin CHEN
- Publication Type:Journal Article
- Keywords:
Nicotine;
NF-kappa B;
Cell adhesion molecules
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the mechanisms of nicotine-induced expression of intercellular adhesion molecule-1(ICAM-1). METHODS: Related luciferase reporter gene plasmids were constructed with molecular cloning techniques;above plasmids and intracontrol plasmid pSV-?-gal were co-transfected into human umbilical vein endothelial cells(HUVECs) with eukaryotic gene transfection techniques; the relative luciferase activities were detected in the transfected HUVECs. RESULTS: Series of luciferase reporter gene containing different sequences of human ICAM-1 promotor and site-directed mutants of NF-?B and Sp-1 in promotor were successfully constructed; Nicotine could increase the expression of luciferase reporter gene plasmid containing-579 bp(pGL3E-579/+36),-230 bp(pGL3E-230/+36) and mutated Sp-1 version(pGL3E-Sp-1-MU)( P
