Effects of LPS on the organization and localization of VE-cadherin and F-actin in cultured endothelial cells
- VernacularTitle:LPS对内皮细胞纤维状肌动蛋白和钙依赖性粘附素的细胞定位和结构的影响
- Author:
Li SONG
;
Qiaobing HUANG
;
Kesen ZHAO
;
Shiwe WANG
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharids;
Endothelial cell;
Actin
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: This experiment is to investigate the effects of LPS on the organization and localization of VE-cadherin and F-actin in cultured human umbilical endothelial cells.METHODS: The human umbilical vein endothelial cell lines ECV-304 were incubated with LPS at different concentrations for 30 min. VE-cadherin was detected by immunofluorescence with primary mAb of VE-cadherin and FITC-conjugated secondary antibody. F-actin was detected with fluorescence staining with rodamine-phalloidin. RESULTS: At high concentration, LPS could induce reorganization of VE-cadherin with the formation of serrata cellular border and enlargement of intercellular gaps, which were apparently different from that in normal conditions with the high fluorescence intensity at cell-cell junction. F-actin depolymerization could also be induced by LPS at high concentration with the formation of stress fiber and filopodia. CONCLUSION: LPS(300 ?g/L) could induce reorganization of VE-cadherin and F-actin in human umbilical vein endothelial cells.
