Inhibition of ET-1 mRNA expression by hammerhead ribozyme in ECV304 cells
- VernacularTitle:核酶抑制内皮素-1 mRNA的表达
- Author:
Huafeng FANG
;
Si JIN
;
Hong YE
;
Dixun WANG
- Publication Type:Journal Article
- Keywords:
RNA, catalytic;
Endothelins;
Gene therapy
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To find out effective hammerhead ribozyme from the designed those in order to inhibit endothelin-1(ET-1) mRNA expression in ECV304 cells. METHODS: The sequences of hammerhead ribozyme were determined by computer. The eukaryotic expression vector pEGFP-R including ribozyme DNA was constructed and delivered into ECV304 cells by liposomes. The expression of ET-1 mRNA was examined by RT-PCR. RESULTS: Ribozyme 145 decreased the expression of ET-1 mRNA in ECV304 cells. CONCLUSION: ET-1 mRNA in ECV304 cells was degraded by R145.
