Whole sequence amplification of an expressed sequence tag in immune cells of F344 rats with severe burns
- VernacularTitle:严重烧伤F344大鼠免疫细胞中一个EST全长序列的扩增
- Author:
Shengjun SHI
;
Zhaofa XIA
- Publication Type:Journal Article
- Keywords:
Burns, Rapid amplification of cDNA ends, Differential expressed gene;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To examine the changes in immune cells which might be involved in systemic immune dysfunction after severe burns, we detected the sequence of expressed sequence tag in lymphocytes and monocytes of F344 rats post-burn. METHODS: Two pairs of primers were designed depending on the sequence of AI764697.1. SMART RACE technology was employed for amplification of whole sequence. Furthermore, the amplified sequence was testified by Northern blot. RESULTS: A 592bp sequence, which was testified by Northern blot, was suceessfally obtained in our experiments. This product was accepted by GenBank and its accession number is AF244895. CONCLUSION: A cDNA whole sequence in immune cells of F344 rats after severe burns was amplified successfully. More research work should be done to identify the resource, location in genome and function of this product.
