Screening and sequencing of the novel differential gene in leptospira serovar lai
- VernacularTitle:赖型钩端螺旋体毒力株新基因差异片段筛选和核苷酸序列分析
- Author:
Jilin ZHAO
;
Lang BAO
;
Huidong ZHANG
- Publication Type:Journal Article
- Keywords:
Leptospira;
Polymerase chain reaction;
Base sequence;
Genes
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To amplify and analyze the differential DNA fragment between pathogenic leptospira serovar lai and nonpathagenic leptospira serovar patoc I. METHODS: The previously subtractive DNA fragment only existing in leptospira serovar Lai was amplified by cassette ligation and semi-nested PCR.The obtained gene was sequenced and searched homologically. In addition, the deduced amino acid was analyzed and the secondary stracture of protein was predicted. RESULTS: The 580 bp DNA fragment, which deposited in GenBank (AF495587), was cloned, and four overlapping open reading frames (ORF) was contained. The high homology with conserved hypothetical protein streptococcus pyogenes was found. CONCLUSION: This study lays foundation for deeply exploring biological actions of new gene and pathogenic mechanism of leptospira serovar lai.
