The activation of JNK may be a signal transduction pathway in MPP~+-induced apoptosis
- VernacularTitle:JNK的激活在MPP~+诱导SHSY5Y细胞凋亡的信号转导中的作用
- Author:
Fang FANG
;
Xiaochun CHEN
;
Yuangui ZHU
;
Yican ZHOU
- Publication Type:Journal Article
- Keywords:
methyl-4-phenylpyridinium;
Neurofibroma;
Apoptosis;
Mitogen-activated protein kinases;
Caspase 3
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the possible signal transduction pathway of 1-methyl-4-phenylpyridinium(MPP +)-induced apoptosis. METHODS: The apoptosis of SHSY5Y cells induced by MPP + was observed by acridine orange-ethidium bromide(AO-EB) staining. Western blot was used to detect the activity of JNK in SHSY5Y cells, and the antisense oligo- neucleutide of JNK were used as inhibitor of JNK. RESULTS: MPP + induced apoptosis in SHSY5Y cells. During the apoptotic process, JNK activity increased. MPP +-induced apoptosis in SHSY5Y cells was obviously inhibited by pretreatment with NAC or by transfection with antisense oligonucleotide of JNK into SHSY5Y cells. Simultaneously, decrease in JNK activity and percentage of positive cleaved caspase-3 cells in these groups were also observed. CONCLUSION: The possible signal transduction pathway of MPP +-induced apoptosis in SHSY5Y cells might be attributed to the production of ROS ,activation of JNK and then activation of caspase 3.
