Effects of angiotensin II on expression of lectin-like oxidized low-density lipoprotein receptor-1 in cultured THP-1 cells
- VernacularTitle:血管紧张素II对巨噬细胞(THP-1细胞)凝集素样氧化低密度脂蛋白受体表达的影响
- Author:
Lei ZHANG
;
Jianhua ZHU
;
Yuanwei HUANG
;
Hangpin YAO
- Publication Type:Journal Article
- Keywords:
Angiotensin II;
Macrophages;
Receptors, LDL
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: Cross talk between oxidized low-density lipoprotein (ox-LDL) and angiotensin II(Ang-Ⅱ) may play an important role in the pathogenesis of atherosclerosis. In this study, we tested the hypothesis that AngⅡ can induce expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), a new receptor for ox-LDL, in cultured THP-1 cells. METHODS: Cultured THP-1 cells were treated with 0.1 ?mol/L PMA for 48 hours at first .Then the cells were incubated with 10 -9 -10 -5 mol/L Ang II for 24 hours, or with 10 -6 mol/L Ang II for various time up to 48 hours. LOX-1 protein expression was examined by cell enzyme linked immunosorbent assay (ELISA), and LOX-1 mRNA was detected by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) . RESULTS: PMA-untreated THP-1 cells didn't express LOX-1 mRNA. After 48 h of PMA treatment, THP-1 cells differentiated and LOX-1 mRNA was detected. Incubation of AngII with THP-1 cells for 24 h significantly increased LOX-1 mRNA and protein expression in a concentration-dependent manner (10 -9 -10 -5 mol/L, P
