Effect of different solution on hypothermic preservation of rat hearts
- VernacularTitle:不同保存液对离体大鼠心脏保存效果的研究
- Author:
Yong ZHANG
;
Rukun CHEN
- Publication Type:Journal Article
- Keywords:
Preservation solution;
Myocardium
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To compare the effects of histidine-tryptophan-ketoglurate(HTK)solution, University of Wisconsin(UW)solution and St.Thomas(ST) Cardioplegia solution Ⅱ on hypothermic preservation of isolated rat hearts. METHODS: Isolated hearts from male Wistar rats were mounted on a Longendorff apparatus to estimate left ventricular end-diastolic pressure(LVEDP),left ventricular developed pressure(LVDP), rate of change of left ventricular pressure(d p /d t max ,d p /d t min ) and coronary flow(CF).Twenty-four rats were randomly divided into three groups: HTK group, UW groups and ST group.They were arrest and stored at 4℃ in each preservation solution for 6 hours. Following storage, they were reperfused and postpreservative function was measured to assess cardiac functional recovery. Frozen samples were analyzed for concentration of adenosine triphosphate. RESULTS: The concentration of adenosine triphosphate in three groups was significantly different( P
