Effect of Liucha extract on tumor K562 cell growth
- VernacularTitle:藏药柳茶提取物对K562肿瘤细胞生长的影响
- Author:
Xin LIU
;
Xingbin PAN
;
Xingy LI
- Publication Type:Journal Article
- Keywords:
Liucha extract;
K562 cells
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of Liucha extract on the growth of tumor cells in vitro and its possible mechanism. METHODS: The capability of colony forming of human leukemia K562 cell in vitro and the cells metabolism were studied by semi-solid agar culture and MTT staining. Then , the changes in morphology in the tumor cells were examined using electronic microscope. RESULTS: Semi-solid agar culture and MTT colorimetric analysis showed that Liucha extrats could significantly inhibit the growth of the tumor cells and their capability of colony forming. Also,under the electronic microscope,it was found that the tumor K562 cell had a narrower perinuclear space,condensation of chromatin and an enlarged mitochondria , in which the cristase disappeared. CONCLUSION: The extract from Liucha possesses an inhibitory effect on K562 cell growth in vitro through affecting the metabolism of the tumor cells.
