Study on DNA extracted from cigarette butts
- VernacularTitle:烟蒂DNA分型的研究
- Author:
Qingxia ZHANG
;
Yacheng LIU
;
Hui TANG
- Publication Type:Journal Article
- Keywords:
Cigarette butt;
PCR;
STR typing
- From:
Chinese Journal of Forensic Medicine
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the DNA extraction and its typing in cigarette butts. Method Chelex 100 extraction method was used to extract DNA of 170 cigarette butts, STR loci were typed after PCR amplification by Profiler Plus kit. Results 21 cigarette butts from one volunteer have no results, the others were all obtained typing results. The samples which were added a little tobacco have no results. Sometimes DNA in sponge contacted with mouth was typed. The small fragment loci were typed in cigarette butts which had been reserved for six months. Conclusion Cigarette butt can be typed by DNA analysis, which is useful in forensic identification.
