Determination of the Content of Compound Tramadol Tablets by HPLC
- VernacularTitle:高效液相色谱法测定复方曲马多片含量
- Author:
Cui CHANG
;
Hongtu YANG
;
Chun DONG
;
Qunying XU
- Publication Type:Journal Article
- Keywords:
HPLC;
tramadol;
nefopam;
content determination
- From:
China Pharmacy
1991;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method to determine the content of compound tramadol tablets METHODS:Stationary phase:Kromasil C18 column,internal standard:phenacetin,mobile phase:methanol-water-triethlamine(55∶45∶0 2),adjusting pH to 4 2 with acetic acid,flow rate∶0 7ml/min,detecting wavelength∶267nm RESULTS:The linear range of tramadol (TR) was 50～500?g/ml(r=0 9 999,n=5) The linear range of nefopam(NFP) was 50～500?g/ml(r=0 9 999,n=5) The average recovery of TR was 99 69% The average content of TR was 100 71%,RSD=0 76%(n=5) The average recovery of NFP was 99 76% The average content of NFP was 99 64%,RSD=0 62%(n=5) CONCLUSION:The method is accurate,rapid and simple
