Prevention of Postoperative Abdominal Adhesions by IL-1? and TNF? Antibody in Mice
- VernacularTitle:应用白细胞介素-1?和肿瘤坏死因子?抗体预防术后腹腔粘连实验研究
- Author:
Shuqiang YUE
;
Kefeng DOU
;
Yanling YANG
- Publication Type:Journal Article
- Keywords:
Interleukin;
Tumor necrosis factor ?;
Peritoneal adhesion;
Disease model
- From:
Journal of Chinese Physician
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the ability of interleukin-1 beta (IL-1?) antibody and tumor necrosis factor alpha (TNF?) antibody to prevent postoperative abdominal adhesion formation in mice.Methods 60 BALB/c mice were randomly divided into four groups: normal saline group(n=15), anti-IL-1? group(n=15), anti-TNF? group(n=15),and anti-IL-1? combined with anti-TNF? group(n=15). After an identical peritoneal injury operation, mice were treated using intraperitoneally with either normal saline or corresponding antibody at the time of abdominal closure. The mice were killed 20 days after operation, the abdominal incisions and the development of intra-abdominal adhesions were observed. Adhesion scoring was based on an overall assessment of the extent,location, and type of adhesion.Results Anti-IL-1? combined with anti-TNF? group had significantly lower adhesion scores (0 84?0 19) than that in other groups(P
