Mechanism by which ganglioside GM1 promotes the production of intracellular amyloid ?-protein
- VernacularTitle:神经节苷脂促进细胞内?-淀粉样蛋白生成的机制
- Author:
Xue WANG
;
Qihua HE
;
Yan RUAN
;
Da ZHANG
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
Gangliosides;
Amyloid beta-protein
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: This research emphasizes the effect of ganglioside GM1 on generation of the intracellular amyloid ?-protein(A?) and investigates where and how GM1 promoted the production of intracellular A?, particularly the more highly amyloidogenic A? 42 which is basis of senile plaque.METHODS: Human neuroblastoma cells transfected with human amyloid precursor protein (APP) cDNA were used to analyze the effect of the various concentrations of GM1 on the level of intracellular total A? by IP-Western blot. Subcellular compartment localized and colocalized with intracellular A? 42 was determined by double or triple immunofluorescence labeling.RESULTS: The intracellular total A? was promoted by GM1, and the levels of intracellular A? were correlated to the concentrations of GM1 in a dose-dependent fashion ( P
