Protective effect of onychin on the human umbilical vein endothelial cells injured by menadione
- VernacularTitle:金粉蕨素对甲萘醌损伤人脐静脉内皮细胞的保护作用
- Author:
Yu GUO
;
Bingyang ZHU
;
Fengxiang YAN
;
Honglin HUANG
;
Duanfang LIAO
- Publication Type:Journal Article
- Keywords:
Onychin;
Endothelial cell;
Vitamin K;
Nitric-oxide synthase
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO - 2/NO - 3 concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO - 2/NO - 3 concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway.
