Effect of human chorionic gonadotrophin on vascular epithelial growth factor expression in JEG-3 cell line
- VernacularTitle:人绒毛膜促性腺激素对JEG-3细胞表达VEGF的影响
- Author:
Zhanjun PANG
;
Fuqi XING
- Publication Type:Journal Article
- Keywords:
Trophoblast;
Neoplasm invasiveness;
Chorionic gonadotrophin;
Endothelial growth factors
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To uncover the effect of human chorionic gonadotrophin (hCG) on vascular epithelial growth factor (VEGF) expression in trophoblasts. METHODS: The semi-quantitative method of reversely transcription polymerase chain reaction (RT-PCR) was employed to investigate the expression of VEGF in trophoblast-derived JEG-3 cell line. RESULTS: VEGF 121 , VEGF 165 and VEGF 189 was shown to express in JEG-3 cells. After incubated with 0, 50, 500, 5 000 , 25 000 U/L hCG for 48 hours respectively, the expression of VEGF (including its 3 isoforms: VEGF 121 , VEGF 165 , VEGF 189 ) was induced gradually with the concentration of hCG increased. Furthermore, VEGF mRNA expression in JEG-3 cells incubated with 25 000 U/L hCG experienced a transient increase before a less significant decrease. CONCLUSION: The autocrine of VEGF might be involved in the effect of hCG on trophoblast or trophoblast-derived choriocarcinoma cell proliferation, migration and invasiveness.
