The mechanism by which pancreatic cancer cells acquire drug resistance against 5-FU and gemcitabine
- VernacularTitle:胰腺癌细胞对5氟脲嘧啶和健择的获得性耐药机制的研究
- Author:
Xin SHI
;
Nairong GAO
;
Mingdong HUO
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Gene expression regulation
- From:
Chinese Journal of General Surgery
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism by which pancreatic cancer cells(PCC) acquire drug resistance against 5-FU and gemcitabine. Methods The cytotoxic effects of 5-FU and gemcitabine on PCC ( Panc-1, Mia-Paca-2 andCapan-1) were assessed by using Sulforhodamine B and the expression of anti-apoptotic genes of the Bcl-x L and mcl-1 were analyzed by RNase protection assay and Western blot. Results5-FU and gemcitabine effect cytotoxicity towards PCC. After repeated treatment with 5-FU, the IC 50 values in Capan-1 cells increased by 2.1 fold (P
