Study of the Antigen Retrieval By Microwave and Expression of CD_(44)s and CD_(44v6) Genes Protein in Human Intracranial Metastastic Tumors and Glioblastomas
- VernacularTitle:微波修复CD_(44)s、CD_(44v6)抗原及其在颅内转移瘤与胶质母细胞瘤中表达研究
- Author:
Zhengwen XIONG
;
Yinchen SUN
;
Hong SU
- Publication Type:Journal Article
- Keywords:
Antigen retrieval;
CD 44;
Intracephalic metastasis;
Glioblastoma,Immunohistochemistry
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of antigen retrieval by microwave and the expression of CD 44 s and CD 44v6 in human intracranial metastastic tumor and human glioblastomas, and the relationship between the expression of CD 44 adhesion molecules and the invasion or metastasis of these tumors.Methods After antigen retrieval by microwave-0.1%Triton X-100(MW-T) and buffer solution of microwave-citrate salts(MW-CA), CD 44 s and CD 44v6 were used to detect the expression of CD 44 adhesion molecules in 20 normal human brain tissues, 35 human glioblastomas, and 30 intracranial metastastic tumor by microwave-LSAB immunohistochemical staining technique.Results Integral optical density(IOD) value of CD 44 s and CD 44v6 positive cells restored by MW-T and MW-CA was higher significantly than that of without antigen retrieval (P
