Effects of Phentolamine on the Function of Isolated Islets of Normal Rats
- VernacularTitle:酚妥拉明对离体正常大鼠胰岛功能的影响
- Author:
Xiaogang WENG
;
Qinggui ZHANG
;
Jingfang DOU
- Publication Type:Journal Article
- Keywords:
Phentolamine;
Isolated islets;
Insulin;
Glucagon;
Rats
- From:
Journal of Chinese Physician
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of phentolamine on the function of the islets isolated from normal rats.Methods The SD rats islets were isolated by digestive method with collagenase and DNase.Millipore Multiscreen Assay System was used to observe the effects of phentolamine on the function of isolated islets. Insulin and glucagon were measured by RIA. Results ⑴Insulin secretion of the isolated islets incubated in culture medium was glucose concentration-dependent manner. Phentolamine had stimulate insulin secretion significantly. Nifedipine (Ca 2+ channel blocker) and diazoxide (K + channel opener) had inhibit the insulin release, but their effects were abolished by phentolamine. ⑵Glucagon secretion of the isolated islets in culture medium incubated was inhibited by glucose. Phentolamine inhibited glucagon secretion significantly, and the inhibiting effect was also concentration-dependent manner,both the nifedipine and diazoxide had the effect too. Conclusion Phentolamine could stimulate insulin secretion, and inhibit glucagon secretion in the isolated islets.It maybe has some hypoglycemic effects on diabetes.
