Effect of Pteris Semipinnata 5F on Fibroblasts Proliferation and Collagen Synthesis in Systemic Scleroderma Skin
- VernacularTitle:半边旗5F对系统性硬皮病患者成纤维细胞增殖和胶原合成的影响
- Author:
Zuohuan XIAO
;
Tie WU
;
Zhihua WU
;
Peihua ZHANG
- Publication Type:Journal Article
- Keywords:
Scleroderma,systemic;
Fibroblast;
Pteris semipinnata
- From:
Chinese Journal of Dermatology
1995;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of herbal medicine Pteris semipinnata5F on the fibroblasts from systemic scleroderma(SSc)skin and explore new drug for SSc treatment.Methods The SSc skin fibroblasts were cultured as an experimental model and the effects of Pteris semipinnata5F were detected.The cell proliferation was determined by MTT assay,and the collagen production by photometric determination.The mRNA expression for typeⅠandⅢprocollagen was detected by RT-PCR.Results It was shown that Pteris semipinnata5F could not only significantly inhibit the proliferation of the cultured SSc skin fibroblasts,but also significantly reduce the soluble collagen content in the culture medium(P
