Apoptosis and Caspase-3 Expression of Spiral Ganglion Cells in Guinea Pig's Cochlea with Cisplatin Ototoxicity
- VernacularTitle:顺铂致豚鼠螺旋神经节细胞凋亡及Caspase-3活化的研究
- Author:
Min ZHANG
;
Zhaohua LIU
;
Changyou JI
- Publication Type:Journal Article
- Keywords:
Cisplatin Ototoxicity Spiral ganglion Apoptosis
- From:
Journal of Audiology and Speech Pathology
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cisplatin ototoxicity on apoptosis of spiral ganglion cells(SGC), and relation between signal transportation of Caspase-3 and apoptosis of SGC.Methods Forty guinea pigs were randomly divided into 4 groups, and injected cisplatin (1, 2, 4 day group and control group). ABR and AERP (40 Hz Auditory Event Related Potentials) were tested every day after the injection, to find out the threshold changes of guinea pigs. Apoptosis was detected by terminal deoxynucleotidyl transferase(TdT)-mediated deoxyuridine triphosphate(d-UTP) nick end labeling method (TUNEL), and immunohistochemistry method was used to detect Caspase-3 p20 segment of SCGs.Results It was observed that the more cisplatin injected, the worse hearing guinea pigs got, and the damnification of threshold on 8KHz was significantly higher than that on 1 kHz. At the same time, TUNEL staining and the expression of Caspase-3p20 segment rose up gradually, and significant difference was observed compared with control group ( P
