Rapid Identification of Rickettsiae using the Real-Time PCR.
10.4167/jbv.2008.38.4.221
- Author:
Hyo Soon PARK
1
;
Jung Hee LEE
;
Kwang Hoon JIN
;
Won Jong JANG
;
Kyung Hee PARK
;
Yoon Hoh KOOK
;
Seung Hyun LEE
Author Information
1. Department of Microbiology, College of Medicine, Konkuk University, Chugju, Chungchungbuk-Do, Korea. shlee@kku.ac.kr
- Publication Type:Original Article
- Keywords:
groEL gene;
Rickettsia;
Real-time PCR
- MeSH:
Diagnosis, Differential;
DNA;
Fever;
Polymerase Chain Reaction;
Real-Time Polymerase Chain Reaction;
Rickettsia;
Scrub Typhus;
Ticks;
Typhus, Epidemic Louse-Borne
- From:Journal of Bacteriology and Virology
2008;38(4):221-226
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In this study, new real-time PCR method based on the groEL gene was developed and investigated. Four spotted fever group (SFG) strains, four typhus group (TG) strains, and four scrub typhus group (STG) strains were easily differentiated as a distinct entity. This PCR assay was applied to detect Rickettsia DNA from 100 ticks. Twelve Haemaphysalis longicornis ticks were found positive and identified as spotted fever group Rickettsia. This real-time PCR method could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of SFG, TG, and STG in a single reaction.
