Molecular Epidemiologic Investigation of Norovirus Infections in Incheon City, Korea, from 2005 to 2007.
10.4167/jbv.2008.38.4.249
- Author:
Young Woo GONG
1
;
Bo Young OH
;
Hye Young KIM
;
Mi Yeon LEE
;
Yong Hee KIM
;
Jong Myoung GO
;
Jea Mann LEE
;
Hye Sook JEONG
;
Doo Sung CHEON
Author Information
1. Incheon Institute of Health & Environment:18-4, Sinheung-dong 2-ga, Jung-gu, Incheon, Korea. kite1014@korea.kr
- Publication Type:Original Article
- Keywords:
Norovirus;
Gastroenteritis;
Faecal specimens;
RT-PCR;
Genotypes
- MeSH:
Base Sequence;
Capsid;
Gastroenteritis;
Genotype;
Humans;
Incidence;
Korea;
Norovirus;
Open Reading Frames;
Polymerase Chain Reaction;
Sequence Analysis
- From:Journal of Bacteriology and Virology
2008;38(4):249-257
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Noroviruses (NoVs) cause major epidemic gastroenteritis in humans. To obtain the molecular epidemiological information on gastroenteritis sporadic cases in Incheon city, Korea, we analyzed the nucleotide sequences of NoV strains detected during 2005~2007. We performed one step RT-PCR amplifying the open reading frame (ORF) 2 (capsid region) followed by semi-nested PCR from the stool samples from acute gastroenteritis patients from 2005 to 2007. Amplicons of the capsid region of norovirus strains were sequenced and analyzed using MegAlign in DNAstar software. Faecal samples were collected from 6,618 acute gastroenteritis patients during the study period. The incidence of NoV infection was about 10.7% (n=708) among patients with acute gastroenteritis and genotypes of the 320 positive samples were determined by sequence analysis. Sequence comparison of NoV strains revealed that 16 genotypes of GII NoV strains were circulated in Incheon city, from 2005 to 2007. Among norovirus strains, the most prevalent genotype GII/4 was most common 69.7% (223 strains), followed by GII/3 17.2% (55 strains), GII/12 4.4% (14 strains), GII/1 2.2% (7 strains), GII/5 1.6% (5 strains), GII/15 1.3% (4 strains) and 0.6% (2 strains) each of GII/9 and GII/16. The GII-3 strains were most frequently detected in Incheon, 2005. From the phylogenetic analysis of NoV strains, we detected 16 genotypes of GII NoV strains during 2005~2007 in Incheon. Our results suggest that various genotypes of human NoV strains in sporadic case of AGE were circulated in Incheon, Korea.
