The killing effect of herpes simplex virus thymidine kinase gene mediated by retroviral vector on human pancreatic carcinoma cell line
- VernacularTitle:逆转录病毒介导单纯疱疹病毒-胸苷激酶基因对胰腺癌细胞的杀伤作用
- Author:
Yagang ZHAO
;
Biaoyan DU
;
Hongbin ZHANG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Gene therapy;
Retrovirus;
Thymidine kinase
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveToobserve the killing effect of herpes simplex virus thymidine kinase/ganciclovir (HSV tk/GCV) suicide gene therapy system on human pancreatic carcinoma cell line. MethdsHSV tk gene was constructed into a retroviral vector pDOR neo. The recombinant plasmid pDOR tk neo was transfected into the retroviral packaging cell PA317. Finally HSV tk gene was transferred into human pancreatic cancer cell line PC 2. The sensitivity to GCV and bystander effect of PC 2/tk cells was tested in vitro. Antitumor effects were also observed after the administration of GCV in nude mice bearing tumor derived from PC 2/tk cells. ResultsHSV tk gene was successfully integrated into host cell. The killing effect of GCV on PC 2/tk cells was in a dose depandent and time depandent manners. The PC 2/tk cells showed a significant "bystander effect". Intraperitoneal injection of GCV postponed the formation of the implanted tumors. ConclusionPancreatic cancer cells expressing HSV tk can be effectively killed by GCV both in vitro and in vivo.
