Evaluation of diagnostic measures for the detection of tumor metastasis in a nude rat xenograft model of human carcinoma
- VernacularTitle:肿瘤转移检测技术在荷瘤裸鼠转移模型上的应用及比较分析
- Author:
Yan SHEN
;
Shusen ZHENG
;
Ilka VOGEL
- Publication Type:Journal Article
- Keywords:
Colonic neoplasm;
Reverse transcriptase polymerase chain reaction;
Immunohistochemistry;
Carcinoembryonic antigen;
Keratin
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the sensitivity and specificity of reverse transcriptase polymerase chain reaction (RT PCR), immunohistochemistry (IHC) and micropartical enzyme immunoassay (MEIA) in detecting metastasis of human colonic carcinoma (HCC) in a nude rat xenograft model. Methods A metastatic model of HCC in nude rats was established by injecting HT 29 cells, a HCC cell line, into the mesenteric vein of the rats. Hepatic and pulmonary metastasis were detected by RT PCR for cytokeratin 20 (CK20) expression, APAAP immunostaining with monoclonal antibody (McAb) KL 1 (anti cytokeratin) for human CK expression, routine pathology (RP) and MEIA for tissue carcinoembryonic antigen (CEA) determination, respectively. Results The detection rate of HCC metastasis by RT PCR, IHC, RP or MEIA was 73 8%, 64 3%, 50% and 33%, respectively. The detection rate of RT PCR was higher than that of RP (? 2 =5 048, P 0 05). The detection rate of IHC was higher than that of MEIA (? 2 =8 052, P 0 05). RT PCR, IHC and MEIA were all highly specific in detecting HCC cells in nude rats. ConclusionsRT PCR for CK20 expression and APAAP immunostaining with the McAb KL 1 for human CK expression were highly sensitive and specific diagnostic techniques in detecing HCC metastasis. MEIA for tissue CEA determination was highly specific, but with a low sensitivity.
