The polymorphism of mtDNA HVI and the application of multiplex amplification of two mitochondrial DNA region to the species and individual identification
- VernacularTitle:mtDNA—HVⅠ和细胞色素b片段的复合扩增及其法医学应用
- Author:
Hong LIU
;
Hongxia LI
;
Chao LIU
- Publication Type:Journal Article
- Keywords:
Forensic science;
Mltiplex PCR;
Mitochondrial DNA (mtDNA) sequence;
Cytochrome-b;
Individual identification;
Species identification
- From:
Chinese Journal of Forensic Medicine
2000;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discriminate the species and individual identification with mitochondrial DNA (mtD-NA) sequencing in forensic medicine practice. Methods The multiplex PCR of mtDNA loop - D high - variation region and cytochrome- b region were investigated. The PCR products were detected with silver- stain method,followed by analysis of the PCR products with fluorescence sequence technique. Results The presence of two bands (358bp,279bp ) indicated the samples were from human, while only one band of 358bp indicated nonhuman origin. The part of mitochondrial DNA loop - D high - variation region (15997 ~ 16236) from 131 unrelated individuals of Guangdong population were sequenced. In all of these samples there were 69 nucleotide variations and 67 haplo-types.There was 2.679 mutation sites on average per person. The polymorphism was 97.92% . Conclusion The methods described here are reliable and very useful in species and personal identification of degraded samples.
