Two - step PCR - SSP genotyping for HLA - A locus and its comparison with serological typing results
- VernacularTitle:HLA—A基因座分步PCR—SSP分型研究
- Author:
Xiaowei LI
;
Huiling LU
;
Lixian/ CHEN
- Publication Type:Journal Article
- Keywords:
Forensic science;
HLA- A locus;
PCR- SSP;
Serological typing
- From:
Chinese Journal of Forensic Medicine
2000;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify routine forensic samples by genotyping method for HLA - A locus. Method A two - step PCR - SSP method was established. The first step is amplification with a pair of primers specific for all HLA - A alleles, the second step is amplification with primers specific for HLA - A30, A31, A33 respectively, using the first step amplification product as template. Secondly amplified PCR products were genotyped by electro-phoresis. Results 100 blood stains with a serological typing result of HLA - A30, A31 and A33 were tested with this method. The discrepancy rate between serological and genetic typing was 29%. Seminal stains, salivary stains reserved for 2 years and blood stains reserved for 18 years in room temperature gave satisfactory results. Conclusions It is better to replace serological typing by genetic typing. A two - step PCR - SSP genotyping method can be applied to forensic samples.
