The effect of hypoxia on proliferation of cultured bovine retinal pigment epithelial cells and expression of the antiapoptotic protein bcl-2
- VernacularTitle:缺氧对牛视网膜色素上皮细胞增生和凋亡基因bcl-2表达的影响
- Author:
Yunping LI
;
Luosheng TANG
;
Songbai JIA
- Publication Type:Journal Article
- Keywords:
Pigment epithelium of eye;
Cells culture;
Cell death;
Cell hypoxia;
Genes,bcl-2
- From:
Chinese Journal of Ocular Fundus Diseases
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of hypoxia on proliferation of cultured bovine retinal pigment epithelium (RPE) cells and expression of the antiapoptotic protein bcl-2. Methods The bovine RPE cells were cultured under normal and hypoxic chamber respectively. After 24 hours, the proliferation of RPE cells was evaluated by[3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromide, MTT]-test. At the same time, anti-bcl-2 protein antibody was examined by immuno-histochemistry method. Results The A value in the hypoxia group was higher than that in the normal group after 24 hours (P
